RURAL DEVELOPMENT ADMINISTRATION);대한민국(농촌진흥청장);REPUBLIC OF KOREA(MANAGEMENT ;REPUBLIC OF KOREA(MANAGEMENT : RURAL DEVELOPMENT ADMINISTRATION)
发明人:
ROH, EUN JUNG,노은정,HEU, SUNG GI,허성기,JUNG, KYU SUK,정규석,KIM, SE RI,김세리,YUN, JONG CHUL,윤종철
申请号：
KR1020110136443
公开号：
KR1020130068958A
申请日:
2011.12.16
申请国别(地区):
KR
年份:
2013
代理人:
摘要：
PURPOSE: Staphylococcus pasteuri RSP-1 which suppresses growth of harmful bacteria, especially antibiotic-resistant Staphylococcus aureus RSP-1 is provided to reduce loss of agricultural products, and to effectively control antibiotic-resistant microorganisms. CONSTITUTION: Staphylococcus pasteuri RSP-1(KACC 91660P) is provided. Probiotics contains the strain, a culture liquid or culture thereof, or antagonist isolated from the culture liquid or the culture. A method for isolating the antagonist from the strain comprises: a step of culturing the strain and centrifuging to collect a culture supernatant； a step of adding hydrophobic resin to the supernatant and adsorbing at 20-25 deg. C for 6-10 hours； a step of removing the unabsorbed residual supernatant and washing； a step of diluting with 70% 2-propanol containing 0.1% TFA； and a step of distilling diluted liquid, suspending in Tris-Cl buffer(pH 6-9) and isolating antagonist.본 발명은 식중독균 등의 유해균, 특히 항생제 내성 황색포도상구균의 생장 억제 활성이 뛰어난 신규 균주인 스타필로코코스 파스트리(Staphylococcus pasteuri) RSP-1 (KACC 91660P) 및 이러한 균주, 이의 배양액 또는 배양물, 또는 이의 배양액 또는 배양물로부터 분리된 길항물질을 이용하는 것을 특징으로 하는 식중독균 등의 유해균 억제 방법에 관한 것이다.
