A non-contact vital sign monitoring system transmits wireless signals to the same side of a biological subject via two antennas with different gains, and the two antennas receive two reflected signals from the biological subject with random body movement. Under a proper setup of the two antennas, the two reflected signals can be adjusted by an amplitude and phase adjusting unit to have the Doppler shift components caused by body movement with equal magnitude and out of phase and the Doppler shift components caused by vital signs with different magnitude. Therefore, the random body movement effect can be cancelled based on the relation between the two reflected signals in using the system to monitor the vital signs of the subject.