Obshchestvo s ogranichennoj otvetstvennostyu "Nanolek"
发明人:
Doronin Aleksandr Nikolaevich (RU),Доронин Александр Николаевич (RU)
申请号：
RU2018147304
公开号：
RU0002706544C1
申请日:
2018.12.28
申请国别(地区):
RU
年份:
2019
代理人:
摘要：
FIELD: biotechnologies.SUBSTANCE: invention relates to the biotechnology. Described is a method for preparing a concentrate of a vaccine against influenza containing HPV, where haemagglutinin, neuraminidase and M1 protein genes are obtained from strain A/California/04/2009； haemagglutinin gene is obtained from strain A/Michigan/45/2015, and neuraminidase and M1 protein genes are obtained from strain A/California/04/2009； haemagglutinin gene is obtained from strain A/Novosibirsk/01/2014, and neuraminidase and M1 protein genes are obtained from strain A/California/04/2009； haemagglutinin gene is obtained from strain A/HongKong/4801/2014, and neuraminidase and M1 protein genes are obtained from strain A/California/04/2009； haemagglutinin gene is obtained from strain B/Phuket/3073/13, and neuraminidase and M1 protein genes are obtained from strain A/California/04/2009 or haemagglutinin gene is obtained from strain B/Brisbane/60/2008, and neuraminidase and M1 protein genes are obtained from strain A/California/04/2009. What is presented is an influenza vaccine concentrate containing one of said HPV types.EFFECT: invention enables to obtain HPV influenza virus having high haemagglutination activity； method also increases antigen output； besides, during the method implementation the viability and high productivity of the target proteins are preserved without the use of controlled expression in the HPV influenza cell-producing cells.4 cl, 44 dwg, 15 tbl, 7 exИзобретение относится к биотехнологии. Описан способ получения концентрата вакцины от гриппа, содержащей ВПЧ, где гены гемагглютинина, нейраминидазы и M1 белка получены из штамма A/California/04/2009； ген гемагглютинина получен из штамма A/Michigan/45/2015, а гены нейраминидазы и M1 белка получены из штамма A/California/04/2009； ген гемагглютинина получен из штамма A/Novosibirsk/01/2014, а гены нейраминидазы и M1 белка получены из штамма A/California/04/2009； ген гемагглютинина получен из штамма A/HongKong/4801/2014, а
