The invention concerns chitosan-based biomimetic scaffolds and methods for modulating their intrinsic properties such as rigidity, elasticity, resistance to mechanical stress, porosity, biodegradation and absorbance of exudates. Therefore, the present invention relates to a layered chitosan-based scaffold wherein said layered scaffold comprises at least two fused layers, wherein at least one layer consists of a chitosan nanofiber scaffold membrane and at least one of the other layers of a porous chitosan scaffold support layer. Moreover, the present invention provides a layered chitosan-based scaffold characterized by (i) a good adhesion between the porous and nanofiber layers, (ii) a tuneable porosity of the nanofiber layer by tuning the distance between the nanofibers, (iii) a stable nanofibers and porous morphology even when immersed in water or other solvents and a process for the preparation of such layered chitosan-based scaffold.Finally, the present invention provides the use of the layered electrospun chitosan-based scaffold of the invention or the layered electrospun chitosan-based scaffold produced by the process of the invention as a wound dressing, in tissue engineering or for biomedical applications.L'invention concerne des échafaudages biomimétiques de chitosane et des procédés de modulation de leurs propriétés intrinsèques telles que la rigidité, l'élasticité, la résistance aux contraintes mécaniques, la porosité, la biodégradation et l'absorbance d'exsudats. La présente invention concerne par conséquent un échafaudage de chitosane stratifié, ledit échafaudage stratifié comprenant au moins deux couches fusionnées, au moins une des couches fusionnées comprenant une membrane de nanofibres de chitosane et l'autre couche fusionnée comprenant une couche de support de chitosane, poreuse. La présente invention concerne en outre un échafaudage de chitosane stratifié, caractérisé par (i) une bonne adhésion entre les couches poreuses et de nanofibres, (ii) une
