PROBLEM TO BE SOLVED: To provide a method for creating a ladybug genetically defective in flying ability that can maintain pest insect control effect without reducing reproductive ability and the survival rate of larva depending on advance of generation.SOLUTION: The method is to create a ladybug genetically defective in flying ability and comprises a process for creating a ladybug lineage genetically defective in flying ability by selecting individual ladybugs having low flying ability out of an arbitrary ladybug group depending on flying distances and repeating operations to obtain the next generation through mass mating of the selected individuals, and a process for inter-lineage mating of the created lineages themselves or a process for mating a ladybug genetically defective in flying ability with an individual just after collected from outdoors, and a process for backcrossing the obtained first generation hybrid (F1) with the parent ladybug lineage defective in flying ability.COPYRIGHT: (C)2010,JPO&INPIT【課題】世代の進行によって繁殖能力や幼虫の生存率が低下せず、害虫防除効果が維持できる遺伝的に飛翔能力を欠くテントウムシを作出する方法を提供すること。【解決手段】遺伝的に飛翔能力を欠くテントウムシを作出する方法であって、任意のテントウムシ集団の中から飛翔能力の低いテントウムシ個体を飛翔距離により選抜し、選抜した個体を集団交配して次世代を得る操作を繰り返すことによって遺伝的に飛翔能力を欠くテントウム系統を作出する工程、および作出した系統同士を系統間交配する工程を含む方法、または、遺伝的に飛翔能力を欠くテントウムシと野外から採集した直後の個体を交配する工程、および得られた雑種一代(F1)に親の遺伝的に飛翔能力を欠くテントウムシ系統を戻し交配する工程を含む方法。【選択図】なし