An apparatus is described, for endodontic treatment, whereby the operations for complete removal of connective, vascular and nervous tissues in the pulp cavity and in the root canals can be automatically carried out by chemical and pharmacological disintegration and by employing suitable enzymatic solutions such as trypsin at various degrees of dilution. The use of positive-displacement pumps guarantees that precise quantities of the chemical substances are used in the stages of emptying, disinfection, washing, drying and final filling of the root canals and of the pulp cavity. Temperature control of the fluids in circulation ensures that reaction times of the chemical substances used can be repeated at each cycle.