The invention to a method increasing content mogroside V in Siraitia grosvenorii suspended cells . In the method, 10 - 200 mg/L of a yeast inducer and 50 - 260 mg/L of methyl jasmonate are respectively added on days 5 to 11 o f the culture cycle of Siraitia grosvenorii suspended cells, thereby increasing synthesis and of V, also the amount of mogroside II secreted . The method is beneficial for the market - oriented and large - scale production and quality control management of mogroside V.