A method for producing a hybrid seed using plant of novel cytoplasmic-genic male sterility raphanus sativus line and dna markers for selecting the plant...
SOON KEE SUNG,SUNGGIL KIM,YOUNG SOON AHN,HYO JEONG KIM,CHAE WAN LIM,YOUNG PYO LEE
申请号:
GB201111840
公开号:
GB2478682B
申请日:
2008.04.04
申请国别(地区):
GB
年份:
2012
代理人:
摘要:
The present invention relates to a method for producing hybrid seeds using a novel Cytoplasmic-Genic. Male Sterility (CGMS) Raphanus sativus line plant and DNA markers for the selection of the same, more precisely a novel CGMS (D-CGMS) Raphanus sativus line plant, a method for producing hybrid seeds using the same, chloroplast DNA-based markers for the selection of the D-CGMS Raphanus sativus line plant having the nucleotide sequence represented by SEQ. ID. NO: 5, mitochondrial DNA-based SNP (Single Nucleotide Polymorphism) markers for the selection of the D-CGMS Raphanus sativus line plant located on the 171st nucleotide of the nucleotide sequence represented by SEQ. ID. NO: 12. The D-CGMS Raphanus sativus line plant of the present invention can be effectively used for the production of hybrid seeds owing to its high stability of male sterility. The chloroplast DNA-based markers and mitochondrial DNA-based SNP markers for the selection of D-CGMS Raphanus sativus line plants can be very effective in the selection of D-CGMS Raphanus sativus line plants during breeding process.