There is provided an inexpensive microneedle device for diagnosis of an allergy, enabling one to perform a clear skin test with simple operation for a short period of time in the diagnosis of allergies. The microneedle device (1) comprises: a microneedle substrate (2), microneedles (3) formed on the substrate at a density of 100 to 10000 needles per 1 cm2, being capable of piercing the skin 50 µm to 500 µm deep, and allergen-holding means (4) formed on the microneedles, holding at least one allergen. The microneedles (3) can be prepared by the use of a non-metallic synthetic or natural resin material.