#$%^&*AU2020102148A420201015.pdf#####ADstracy The present invention relates to a purification system, in particular to a zero-emission complex purification system of large-scale culture ponds and a construction method thereof. The system includes a pond culture system, a primary purification system, a secondary purification system, a tertiary purification system and an impounding reservoir which are connected in sequence. The impounding reservoir is connected with the pond culture system through a water supply system. The primary purification system is a purification system including eco-bases. The secondary purification system is a bio-floating bed purification system including aquatic plants. The tertiary purification system is a purification system including biological brushes. The system organically combines the eco-bases, bio-floating beds and biological brushes, and treats nutrient-rich substances in the culture water with the thought of complex treatment to achieve an effect of purifying the culture water. With long-term experiments, through proper cooperation and coordination of various treatment systems, zero emission is realized, and an effective means is provided for ex-situ regulation of the water in the culture ponds. 1