The invention relates, in general, to synthetic chimeric poxviruses, compositions comprising such viruses, and the development and use of systems and methods for producing such synthetic chimeric poxviruses. The synthetic chimeric poxviruses are well suited for live virus vaccines and pharmaceutical formulations.本發明大體上係關於合成嵌合痘病毒、包含此類病毒之組合物,以及用於製造此類合成嵌合痘病毒之系統與方法的開發及使用。該等合成嵌合痘病毒非常適合於活病毒疫苗及醫藥調配物。