BANCHEREAU JACQUES F,KLECHEVSKY EYNAV,ZURAWSKI GERARD,ZURAWSKI SANDRA
申请号：
IN9737/DELNP/2012
公开号：
IN2012DN09737A
申请日:
2012.11.09
申请国别(地区):
IN
年份:
2014
代理人:
摘要：
Immunostimulatory compositions and methods comprising an ITIM motif containing DC immunoreceptor (DCIR) to mediate potent crosspresentation are described herein. The inventors evaluated human CD8+ T cell responses generated by targeting antigens to dendritic cells (DCs) through various lectin receptors. A single exposure to a low dose of anti DCIR antigen conjugate initiated antigen specific CD8+ T cell immunity by all human DC subsets including ex vivo generated DCs skin isolated Langerhans cells and blood mDCs and pDCs. Enhanced specific CD8+ T cell responses were observed when antigens like FluMP MART 1 viral (HIV gag) etc. were delivered to the DCs via DCIR compared to those induced by a free antigen or antigen conjugated to a control mAb or delivered via DC SIGN another lectin receptor. Addition of Toll like receptor (TLR) 7/8 agonist enhanced DCIR mediated crosspresentation as well as crosspriming. Thus antigen targeting via the human DCIR receptor allows activation of specific CD8+ T cell immunity.
