A biological tissue is sutured or ligatured, a ligature wire is cut, and then a holding member is reliably separated. A holding member 23 which holds the ligature wire 20 for ligaturing a pathological lesion portion includes a first cylindrical member 30 and a second cylindrical member 31 fitted to a distal end portion of the first cylindrical member 30. The ligature wire 20 is allowed to pass through a pair of distal lateral holes 42a and 42b formed by the first and second cylindrical members 30 and 31, is drawn out of the outer peripheral side of the first cylindrical member 30, and then is drawn into the first cylindrical member 30 through proximal lateral holes 38a and 38b formed on the proximal side of the first cylindrical member 30.
