METHODE ET COMPOSITION PERMETTANT DE PRODUIRE DES AGENTS REGENERATEURS ET ANTI-INFLAMMATOIRES/ANTI-CATABOLIQUES AMELIORES A PARTIR D'UN LIQUIDE PHYSIOLOGIQUE AUTOLOGUE
Described are methods of producing an autologous composition useful for treatment of damaged and/or injured connective tissues, chronic tendinosis, chronic muscle tears and/or chronic degenerative joint conditions and skin inflammatory disorders in a mammal. The method comprises preparing an anti-inflammatory/ anti-catabolic component of the autologous composition comprising IL-1ra and TIMPs. An anti-inflammatory/ anti-catabolic component is prepared comprising: collecting blood from the mammal； delivering the blood to a tube； incubating the blood at a temperature of from about 37°C to about 39°C for about 24 hours preferably in the presence of sodium citrate； centrifuging the blood to separate the blood into a supernatant component and a cellular fraction； collecting the supernatant component. The method further comprises the step of preparing a regenerative component of the autologous composition comprising: collecting blood from the mammal； delivering the blood to a tube in the presence of about 4% citric acid； centrifuging the blood to separate a platelet rich plasma component from a whole blood； collecting the platelet rich plasma component； and mixing the supernatant component with the platelet rich plasma component to provide the autologous composition. Also provided is a method of treating damaged and/or injured connective tissues, chronic tendinosis, chronic muscle tears and/or chronic degenerative joint conditions and skin inflammatory disorders in a subject with the autologous composition, an autologous composition for treating damaged and/or injured connective tissues, chronic tendinosis, chronic muscle tears and/or chronic degenerative joint conditions and skin inflammatory disorders in a mammal and the use of the autologous composition for the treatment of damaged and/or injured connective tissues, chronic tendinosis, chronic muscle tears and/or chronic degenerative joint conditions and skin inflammatory disorders in a mammal.
