表面プラズモンおよび蛍光共鳴エネルギー転移を利用した免疫アッセイ
- 专利权人:
- 发明人:
- 申请号：
- JP20100544084
- 公开号：
- JP5720248(B2)
- 申请日:
- 2009.12.22
- 申请国别(地区):
- 日本
- 年份:
- 2015
- 代理人:
- 摘要：
Disclosed is an immunoassay utilizing fluorescence resonance energy transfer (FRET) and surface plasmon-field enhanced fluorescence spectroscopy (SPFS). Specifically disclosed is an immunoassay method for measuring the amount of an antigen in a sample using a primary antibody having, conjugated thereto, a fluorescent dye that acts as a donor molecule and a secondary antibody having, conjugated thereto, a fluorescent dye that acts as an acceptor molecule. The immunoassay comprises at least the following steps (a) and (b): (a) allowing an antigen-antibody reaction between the antigen contained in the sample and both the primary and secondary antibodies to occur and immobilizing an immunological complex comprising the antibodies and the antigen sandwiched between the antibodies on a metal thin film support; and (b) irradiating the immunological complex produced in the preceding step with a laser beam by means of SPFS and measuring the quantity of fluorescence emitted from the acceptor molecule as a result o
- 来源网站：
- 中国工程科技知识中心
