A method for quantitative flow analysis of a fluid flowing in a conduit from sequences of consecutive image frames of such a conduit, which image frames are timely separated by a certain time interval, the method comprising:a) selecting a start frame and an end frame from the sequence either automatically or upon user input;b) determining a centreline of the conduit in the start frame;c) determining a centreline of the conduit in the end frame;d) selecting a common start point on the centreline of the start frame and on the centreline of the end frame either automatically or upon user input;e) selecting an end point on the centreline of the start frame;f) selecting an end point on the centreline of the end frame;g) calculating centreline distance between start point and end point of the start frame;h) calculating centreline distance between start point and end point of the end frame;i) calculating a local flow velocity as a function of centreline distances and time interval between start frame and end frame.A corresponding device and computer program are also disclosed.