A method of improving the accuracy of calibrating a fluidic system, comprises providing a system for detecting an analyte in a bodily fluid from a subject comprising a fluidic device for providing the bodily fluid, the fluidic device having a calibration assembly and a reader assembly for detecting the presence of the analyte, measuring one or more parameters that are fitted to a calibration curve associated with the fluidic device, comparing the one or more parameters with predetermined parameters associate with the fluidic device, and adjusting a signal output by the ratio of the predetermined parameters to the one or more parameters. The fluidic device also has a sample collection unit and an assay assembly where the sample collection unit allows the bodily fluid to react with reactants contained within the assay assembly.
