The present invention relates to medical biotechnology and cell technology. Proposed is a method for isolating a stromal vascular fraction of adipose tissue, which includes: decantation of a lipoaspirate； delicate washing out of the lipoaspirate in buffer solutions； enzymatic treatment by using a mixture of collagenases, thermolysin, dispase and trypsin at a temperature of 37ºC； centrifugation at 500-2000 g and filtration through microfilters with a pore size of 10-300 microns with the subsequent removal of fat and stromal tissue matter； repeated washing out and concentration of the cell fraction, wherein all the stages of the process are carried out under closed system conditions. A corresponding device is configured as a sealed container consisting of two chambers, which are separated from one another by nylon microfilters with pore sizes of 10-500 microns. The device comprises fittings provided with valves, as well as channels for the introduction and removal of biological tissue and a cellular end product for subsequent administration. Thanks to the presence of a cocktail of mature and progenitor cells in the stromal vascular fraction, which have a paracrine effect, the sterility of all the stages of the process and the safety thereof, the claimed method can be used in medicine for cell-based therapy and regeneration of organs and tissues, including: soft tissue and bone regeneration, treatment of cosmetic defects, chronic trophic and radiation ulcers, burns, Crohn's disease, multiple sclerosis, graft-versus-host reactions, myocardial infarction and strokes of different origins.
