There is provided a method of producing a reagent tape, that comprises a base tape layer and a series of consecutive and separate reagent pads of a reagent, for use in a milk sampling device. The milk sampling device is arranged to supply a droplet of a milk sample onto one of the reagent pads on the tape in order to produce a response in the reagent to detect a presence or concentration of a substance in the milk sample. The method comprises providing the base tape layer of the reagent tape, applying onto the base tape layer a continuous layer of the reagent, dividing the supplied continuous reagent layer into separate reagent pads by providing a hydrophobic barrier line between said pads. This may be done by laser ablating thin lines of reagent material, or by providing additional hydrophobic material int othe layer. Thus, a simple and fast way of providing a tape with pads is obtained, in which a narrow spacing between pads is possible. The invention also provides a tape made by the method, and a milking device with a sampling device with such a tape.
