DURACK, GARY,HATCHER, JEREMY T.,WESTFALL, LON A.,HELBING, DAVID R.,WALLACE, JEFFREY D.,DIDION, BRADLEY,NAYAK, NIRAJ V.,ANZAR, MUHAMMAD,LUDWIG, CINDY L.,GRAHAM, JEFFREY A.,CROWLEY, KATHLEEN S.,VANDRE,
申请号：
ES10184097
公开号：
ES2534395T3
申请日:
2004.03.29
申请国别(地区):
ES
年份:
2015
代理人:
摘要：
A system for classifying sperm cells X and Y, the system being characterized by including: a system of fluid supply for supplying a variable rate fluid stream containing X and Y sperm cells one apparatus flow cytometry to: (1) receiving the flow and form droplets containing sperm cells, including the first droplets droplets each containing one or more sperm cells X, second droplets each containing a or more sperm cells Y, and a third plurality of droplets each containing one or more X sperm cells and one or more sperm cells Y (2) sorting the first droplets between the second and third droplets (3) collecting the first drops to provide at least a population of sperm cells X and (4) identify a number of X sperm cells in at least one population and responsive to instructions received from the apparatus flow cytometry to vary the rate at which fluid is supplied to the apparatus flow cytometry as a function of the amount of sperm cells X identified in said at least controlling a population regarding the total number of X cells in the first, second and third droplets, and wherein the control is functional to increase the rate of fluid delivery when the number of X cells in said at least one population is above the acceptable amount and to decrease the rate of fluid delivery when the number of X cells in said at least one population is below the acceptable amount.Un sistema para clasificar células espermáticas X e Y, estando el sistema caracterizado por que incluye: un sistema de suministro de fluido de tasa variable para suministrar una corriente de fluido que contiene células espermáticas X e Y una aparato de citometría de flujo para: (1) recibir la corriente y formar gotitas que contienen las células espermáticas, incluyendo las gotitas primeras gotitas que contienen, cada una, una o más células espermáticas X, segundas gotitas que contienen, cada una, una o más células espermáticas Y, y una tercera pluralidad de gotitas que contienen, cada una, una o más células esperm
