THE SCRIPPS RESEARCH INSTITUTE;REISFELD, RALPH, A.;XIANG, RONG;LUO, YUNPING
发明人:
REISFELD, RALPH, A.,XIANG, RONG,LUO, YUNPING
申请号：
WO2007US21414
公开号：
WO2008054612(A3)
申请日:
2007.10.05
申请国别(地区):
世界知识产权组织国际局
年份:
2008
代理人:
摘要：
A DNA composition useful for eliciting an immune response against tumor-associated macrophages comprises a DNA construct that encodes for a cysteine endopeptidase (e.g., legumain) or a polypeptide comprising at least one immunogenic fragment thereof that is overexpressed in tumor-associated cells such as tumor-associated macrophages, or an immunogenic fragment thereof that is expressible in immune cells, and which is incorporated in a pharmaceutically acceptable carrier. In preferred embodiments, the composition encodes a polypeptide comprising a plurality of immunogenic fragments of a tumor associated cysteine endopeptidase (e.g., legumain) joined together serially such that each fragment is connected to at least one other fragment by a linker peptide such as AIaAIaAIa or AlaAlaTyr. In one preferred embodiment, the composition also includes a DNA construct that encodes an immune effector protein, such as a cytokine. The DNA composition of the invention can be used alone or in combination with a chemotherapeutic agent to treat diseases such as tumors and tumor metastases.
