김태웅,성재모,박형진,조재열,박영철,성기호,KIM, TAE WOONGKR,SUNG, JAE MOKR,PARK, HYUNG JINKR,CHO, JAE YOULKR,PARK, YOUNG CHULKR,SUNG, GI HOKR
申请号：
KR1020130088704
公开号：
KR1020150014033A
申请日:
2013.07.26
申请国别(地区):
KR
年份:
2015
代理人:
摘要：
The present invention relates to an inhibition effect of a water extract of Cordyceps militaris (CMWE) which is a novel anti-cancer functional substance on hepatocellular carcinoma cells, which is investigated through various experiments. The present invention provides biopharmaceuticals as antitumor agents. A method for preparing the CMWE comprises the steps of: extracting and purifying a functional substance from Cordyceps militaris； cultivating human hepatoma cell line (HepG2) and human liver cell line (Chang) which is normal cells； treating the human liver cell line with hepatotoxicity inducing substance, cultivating the same, and measuring cell viability； analyzing using high performance liquid chromatography (HPLC)； and analyzing using flow cytometry and performing western blotting to investigate biochemical mechanisms of protection effects of CMWE on liver cell damages.본 발명은 지금까지 알려지지 않은 신규 항암기능성 물질인 번데기동충하초 물추출물인 CMWE를 제조하고 상기 화합물의 간암세포 억제 효과를 다양한 실험을 통해 규명한 발명에 관한 것으로 항암제로서 생물 의약 소재를 제공하는 뛰어난 효과가 있다.
