КЛИНИК ЮНИВЕРСИТЭР СЕН-ЛЮК (BE);ЮНИВЕРСИТЕ КАТОЛИК ДЕ ЛУВЕН (BE)
发明人:
ДЮФРАН Дени (BE),ДЕЛЛУА Кристьян (BE)
申请号：
RU2011153226/15
公开号：
RU2011153226A
申请日:
2010.06.04
申请国别(地区):
RU
年份:
2013
代理人:
摘要：
1. Biomaterial having a multidimensional structure containing differentiated MSCs tissue and a demineralized bone matrix, which is dispersed within the differentiated MSCs tissue. The biomaterial of claim 1, wherein the MSCs used to form differentiated tissue MSCs are of human or animal origin. The biomaterial of claim 1, wherein the MSCs are late passage derived stem cells from adipose tissue. The biomaterial according to claim 1, which is three-dimensional. The biomaterial according to claim 1, characterized in that it contains osteocytes coated with a mineral membrane, and connective tissue, including collagen. The biomaterial according to claim 1, characterized in that it contains chondrocytes, an extracellular matrix containing collagen and proteoglycans. The biomaterial according to claim 1, wherein the demineralized bone matrix is in the form of particles having an average diameter of 50-2500 microns. An implant containing biomaterial according to any one of claims 1 to 7.9. A method for producing multidimensional biomaterial, including incubating MSCs in an osteoblastic and or chondrogenic medium for 15-25 days, then adding demineralized bone matrix to the medium and continuing the incubation for another 15-30 days. 10. The method according to claim 9, wherein the osteoblastic medium is a standard medium supplemented with human serum, dexamethasone, sodium ascorbate, sodium dihydrogen phosphate, penicillin and streptomycin. The method of claim 9, wherein the chondrogenic medium is a standard medium supplemented with human serum, dexamethasone, TGF-B3, L-proline, sodium ascorbate, sodium dihydrogen phosphate, sodium pyruvate, insu1. Биоматериал, имеющий многомерную структуру, содержащий дифференцированную MSCs ткань и деминерализованный костный матрикс, который диспергирован внутри дифференцированной MSCs ткани.2. Биоматериал по п.1, в котором MSCs, применяемые для формирования дифференцированной MSCs ткани, имеют человеческое или животное происхождение.3. Би
