Biogen Idec MA Inc.;Massachusetts Institute of Technology
发明人:
Vlijmen, Herman Van,Sherman, Brian Woody H.,Lugovskoy, Alexey Alexandrovich
申请号：
AU2011253611
公开号：
AU2011253611B2
申请日:
2011.11.23
申请国别(地区):
AU
年份:
2012
代理人:
摘要：
#$%^&*AU2011253611B220120712.pdf#####ABSTRACT The invention relates to methods of modulating the antigen-binding affinity of an antibody by determining, using data corresponding to the structure of a complex between the antibody and an antigen in a solvent, a representation of a charge distribution of the CDRs of the antibody which minimizes electrostatic contribution to binding free energy between the antibody and the antigen in a solvent. Guided by these determinations, the antibody is accordingly modified (altered) to improve upon, e.g., antibody/antigen binding by modifying at least one amino acid residue to decrease the binding free energy between the antibody and antigen when bound in a solvent.
