Disclosed is a method for producing a non-transgenic, herbicide resistant or tolerant plant comprising introducing into plant cells a recombinagenic oligonucleobase with a targeted mutation in the EPSPS gene to produce plant cells with a mutant EPSPS gene that expresses a EPSPS protein that is mutated at amino acid position Val114 in an Arabidopsis EPSPS protein (AF360224) and optionally further mutated at one or more amino acid positions selected from the group consisting of: Thr179 and Pro183； selecting a plant cell exhibiting improved tolerance to glyphosate as compared to a corresponding wild-type plant cell； and regenerating a non-transgenic herbicide resistant or tolerant plant having a mutated EPSPS gene from said selected plant cell. Also disclosed are the herbicide resistant plants that result from the disclosed method.
