A perineum overlay device (100) for locating anatomical features within theperineum of a patient and/or needle insertion sites for targeting anatomicalfeatures within theperineum of a patient includes a section of flexible material (102) and aplurality of markings(104) printed on a first side (106) of the section of the material. Thesection of flexible materialis configured to conform and cover a portion of the perineum of a patient. Theplurality ofmarkings are each configured to identify a location of an anatomical feature(104A-G) within theperineum and/or a needle insertion site (104N) corresponding to a targetedanatomical featurewithin the perineum, when the section is positioned over the perineum of apatient.[0048] In amethod of treating, a pelvic condition of a patient. a perineum overlay device(100) comprising a section of flexible material (102) is positioned over theperineum (144) of apatient. In some embodiments, alignment markings (104) on a first side (106)of the section arealigned (170) with corresponding features on the perineum. A second side (140)of the section isbonded (172) to the perineum. A needle (150) is inserted (174) through aneedle insertion sitemarking (104N) or an anatomical marking (104A-G) on the first side of thesection and intotissue (152) of the patient. A pelvic treatment is then performed (176) usingthe needle.