The Children's Hospital of Philadelphia;The Childrens Hospital of Philadelphia
发明人:
High, Katherine A.,Wright, John Fraser,Hauck, Bernd,Qu, Guang
申请号：
AU2012352394
公开号：
AU2012352394B2
申请日:
2012.12.12
申请国别(地区):
AU
年份:
2017
代理人:
摘要：
In accordance with the present invention, a method for increasing the yield of rLV vector particles comprising a trans gene encoding a therapeutic protein or fragment thereof is disclosed. In one approach, cells are transfected with plasmids encoding the necessary components for rLV production using a calcium chloride transfection mix at pH 7.1 wherein the calcium chloride and plasmids form a complex which is added to the cells at a constant speed. The cells are then incubated for a suitable time period wherein virus particle media is collected at least twice during the incubation period and stored in a cold storage unit, thereby reducing virus inactivation.
