Provided is a method for obtaining high yield stable expression cell clones from myeloma cell lines in a protein free culture medium. The method is used for industrial production of a recombined antibody and includes three stages: (1) adapting to a protein free culture medium statically culturing cells at a low density and gradually reducing a fat rich supplement to a chemical culture medium (2) adapting to the protein free culture medium: culturing cells at a high density and using a perfusion fermentation system in a laboratory scale and (3) screening high yield stable expression cell clones from the cells after fermentation ends. The cell clone may be used to produce a humanized anti NeuGcGM3 14F7h recombined antibody.
