Described herein is a reliable method for preparing a potent vaccine useful for immunotherapy comprising the step of cryopreserving a population of cells undergoing immunogenic cell death, and using such cells to activate dendritic cells for use in immunotherapy. In a specific embodiment, the method comprises cryopreserving cancer cells undergoing cell death, which can be used to prepare a pharmaceutical composition for immunotherapy against cancer.本文闡述製備可用於免疫療法之有效疫苗的可靠方法，其包含以下步驟：冷凍保存進行免疫原性細胞死亡之細胞群體，及使用該等細胞活化用於免疫療法中之樹突細胞。在具體實施例中，該方法包括冷凍保存進行細胞死亡之癌細胞，其可用於製備用於對抗癌症之免疫療法之醫藥組合物。
