A cell vitrification cryopreservation device, comprising: a straw device, a pre-freezing device, a freezing unit, a driving device, a control unit (40), and a holding platform (52) for holding a cell carrier (70). The straw device is driven by and connected to the driving device. The driving device is signal-connected to the control unit. The straw device comprises a straw (22) for obtaining a cell from the cell carrier. The control unit is for controlling the driving device so as to drive the straw device to obtain the cell and to transfer the cell into the freezing unit. The pre-freezing device pre-freezes the straw (22) during the process of transferring the obtained cell into the freezing unit by the straw device. The cell vitrification cryopreservation device achieves fully automatic operation, reduces manual operation error in the prior art, facilitates batch processing, and prevents the need for an operator to handle liquid nitrogen in close proximity so as to enhance safety. A cell vitrification cryop
