A needle-free connector including a guiding base, a connecting base, a valve member, and a seal member is provided. The guiding base has a conduit, and the conduit extends downward along an axial direction. The connecting base is disposed on the guiding base and has a screw portion and a passage running through the screw portion. A chamber is formed between the guiding base and the connecting base, and the conduit and the passage respectively communicate with the chamber along the axial direction. The valve member is disposed between the guiding base and the connecting base and is movably located in the chamber. The seal member is disposed at an end of the valve member far away from the conduit, and the seal member and the valve member are adapted to be connected into a whole in a manufacturing process.