Disclosed is an optical needle comprising a needle body, a coupling seat and an optical fiber. The needle body comprises a cavity to accommodate a portion of the optical fiber. The coupling seat provides a junction plane to couple a light source. An end of the needle body is aligned with the junction plane, so to align an end of the optical fiber with the junction plane. The optical needle is characterized in that the long axis of the optical fiber reduces from a section aligned with the junction plane to an end adjacent to the needle body. The coupling interface may be provided in a recess to accommodate the light source. The optical needle may associate a light source. A method for preparation of the optical needle is also disclosed.