The present invention relates to a diaphragm, and stethoscopes provided with the same. The diaphragm having a plate-shaped vibratable portion, and the vibratable portion has a first surface. The diaphragm further comprises a compliant structural layer disposed on the first surface. When the diaphragm is assembled with a sound collecting head, the compliant structural layer is in contact with the sound collecting head to enhance the airtightness between the diaphragm and the sound collecting head to prevent the acoustic vibration energy from dissipating. The invention also relates to methods for manufacturing the diaphragm which can quickly produce a large number of diaphragms by simple processes.