A novel anti-PD-1 antibody that specifically binds to cell surface PD-1 is provided. Nucleic acid molecules encoding anti-PD-1 antibodies are also provided for expression vectors and host cells for expressing anti-PD-1 antibodies. The invention further provides a method for producing an anti-PD-1 antibody and use thereof.提供了與細胞表面PD-1特異性結合的新型抗PD-1抗體。還提供了編碼抗PD-1抗體的核酸分子,用於表達抗PD-1抗體的表達載體和宿主細胞。本發明進一步提供了產生抗PD-1抗體的方法及其用途。