The present solution includes a 3D printer designed to meet clean room requirements. The printer can be configured for 3D bioprinting. The printer can be used to create cellular scaffolds, tissue grafts, and organs. The printer can include a sterile (or clean room-like) environment for the printing of items. The printer can include an enclosure that isolates the manufacturing processes from the external environment and that can be sterilized between printing runs. The solution also includes a printing kit that can be independently sterilized and passed into the system's enclosure.