БАДЕР Мартин (DE),БЕНЕР Моника (DE),ЙОХНЕР Антон (DE),КЕТТЕНБЕРГЕР Губерт (DE),МОЛЬ Зильке (CH)
申请号：
RU2013111676/15
公开号：
RU2013111676A
申请日:
2011.08.25
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
1. A process for preparing lipid particles consisting of a polypeptide, characterized in that said lipid particle is formed in the presence of Zwittergent 3-8 or Zwittergent 3-10.2. A method according to claim 1, characterized in that said polypeptide is incubated with Zwittergent for about 2 hours to 60 p.3. A method according to claim 1, characterized in that it comprises the following steps: i) preparing a first solution comprising a denatured polypeptide, ii) adding the first solution to the second solution which comprises at least one lipid and Zwittergent 3-8 or Zwittergent 3- 10, but is free of said polypeptide, and iii) removal of Zwittergent solution obtained in step ii) to obtain a lipid chastitsy.4. A method according to claim 1, characterized in that it comprises the following steps: i) preparing a solution containing native polypeptide, ii) adding at least one lipid and Zwittergent 3-8 to Zwittergent 3-10 or solution of i), and iii) removing Zwittergent from the solution obtained in step ii) to obtain a lipid chastitsy.5. A method according to claim 1, characterized in that said polypeptide has an amino acid sequence selected from amino acid sequences of SEQ ID NO: 01, 02, 04-52, 66 or 67, or it comprises at least a continuous fragment thereof comprising at least 80% amino acid sequence of SEQ ID NO: 01, 02, 04-52, 66 or 67.6. A method according to claim 5, characterized in that said polypeptide is tetranektin-apolipoprotein AI, comprising the amino acid sequence of SEQ ID NO: 01, or SEQ ID NO: 02, or SEQ ID NO: 66, or SEQ ID NO: 67.7. A method according to claim 3, characterized in that at least one lipid is a two lipida.8. A method according to claim 7, characterized in that said lipids are ET1. Способ получения липидной частицы, включающей полипептид, отличающийся тем, что указанная липидная частица образуется в присутствии Zwittergent 3-8 или Zwittergent 3-10.2. Способ по п.1, отличающийся тем, что указанный полипептид инкубируют с Zwittergent в теч
