Methods of producing autologous composition useful for the treatment of damaged and / or injured connective tissues, chronic tendonitis and / or chronic degenerative joint conditions, and chronic inflammatory skin diseases in mammals are described. The method comprises preparing an antiinflammatory / anticatabolic component of the autologous composition comprising il-1ra and timps. An anti-inflammatory / anticatabolic component is prepared comprising: collecting mammalian blood； blood supply to the tube； incubating the blood at a temperature from about 37 ° C to about 39 ° C for about 24 hours preferably in the presence of sodium citrate； centrifuging blood to separate blood into a supernatant component and a cell fraction； collection of the supernatant component. the method further comprises the step of preparing a regenerating component of an autologous composition comprising collecting mammalian blood； blood supply to a tube in the presence of about 4% citric acid； centrifuging blood to separate a planet-rich plasma component from whole blood； collecting the platelet rich plasma component and mixing the supernatant component with the platelet rich plasma component to provide the autologous composition. Also provided is a method for treating damaged and / or injured connective tissues, chronic tendinosis, chronic muscle injuries and / or degenerative joint conditions, and inflammatory skin diseases in an individual with the autologous composition, an autologous composition for treating damaged and / or connective tissue. / or injured, chronic tendinosis, chronic muscle injuries and / or chronic degenerative joint conditions and inflammatory skin conditions in a mammal and the use of autologous composition for the treatment of damaged and / or injured connective tissues, chronic tendinosis, chronic muscle injuries and / or chronic degenerative joint conditions and inflammatory skin conditions in a mammal.são descritos métodos de produção de composição autóloga útil
