#$%^&*AU2016101561A420161222.pdf#####ABSTRACT The present invention provides a gene encoding truncated human keratinocyte growth factor. The gene is any one selected trom the group consisting of, 1) the sequence of SEQ ID No: i in the sequence list； 2) a DNA sequence having a sequence honology of 90% or more to the DNA sequence of SEQ ID No: I and encoding proteins with the same functions as that encoded by SEQ ID No: L Also provided is a method of preparing a truncated human keratinocyte growth factor, comprising the following Steps: (1) preparation of plasmids by using a sequence of a fusion gene of a truncated human keratinecyte growth factor and a mature signal peptide, prepared through artil total gene synthesis； (2) obtaining a product by the steps of fermentation. cultivation. induction and expression, The method according to the Oresent invention is simple and can efficiently express genetic recombinant truncated human keratinocyte growth factor. The expression quantity of the truncated hunan keratinocyte growth factor in a fermenter is 250mg /L or more, w hich is more than three times of that of the wild type. Figure IDrawing BamHI (933) .'AO 1 a-factor ig'KGFM3 NotI (1631) 3'AOX1(TT) pPIC9K- 'i-KG F A23 9672bp 3'A OX 1 -H S H3I4 SaI(3573) Figure 1 500~bp+ Figure 2 1
