WANG, Yong,汪勇,REANEY, Martin J. T.,雷尼·马丁·J. T.,LI, Xiaofeng,李小凤,LIAO, Wenzhen,廖文镇,LIANG, Shan,梁珊,TENG, Yinglai,滕英来,SHIM, Youn Young,沈允英,BURNETT, Peta-Gaye Gillian,伯奈特·佩塔-盖伊·吉莉安
申请号：
CNCN2016/088048
公开号：
WO2017/005134A1
申请日:
2016.06.30
申请国别(地区):
WO
年份:
2017
代理人:
摘要：
Disclosed are a preparation method and use of a linseed polysaccharide having an antiviral activity and an immunological activity, comprising: using a linseed as a raw material obtaining a crude linseed polysaccharide by grinding the linseed, separating a husk from the linseed, performing microwave-aided hot water extraction, using a Sevage treatment method to remove protein, performing ethanol precipitation, and lyophilization and employing ion-exchange column chromatography, sephadex column chromatography and ultrafiltration to obtain a linseed polysaccharide having a triple-helical structure. FHP-1 of the invention is homogeneous and has a molecular weight of 2626 kDa. The invention makes a breakthrough to separate a polysaccharide having a triple-helical structure from the linseed, and a cytobiological experiment proved that the polysaccharide can reduce expression of a hepatits B surface antigen and a hepatitis B e antigen expression, suppress replication of a hepatitis B virus (HBV) DNA, activate an immune response, and facilitate secretion of a tumor necrosis factor (TNF-a), interleukins IL-6 and IL-12, and an inflammatory factor NO by an immune cell. The linseed polysaccharide having the triple-helical structure is applied to functional food, such as anti-virus drinks or yoghurt, thereby realizing prevention of HBV infection, treatment of an immune defect and an improvement of immunity.La présente invention concerne un procédé de préparation et dutilisation dun polysaccharide de graine de lin présentant une activité antivirale et une activité immunologique, comprenant : lutilisation dune graine de lin en tant que matière première lobtention dun polysaccharide de graine de lin brut par broyage de la graine de lin, la séparation de lenveloppe de la graine de lin, lexécution de lextraction à leau chaude par micro-ondes, lutilisation dun procédé de traitement Sevage afin de retirer la protéine, lexécution de la précipitation à léthanol, et la lyophilisation et
