A recombinant baculovirus displaying on its envelop a fusion protein is disclosed. The fusion protein comprises a heterologous antigen, and a C-terminal region of baculovirus envelope GP64 protein, which has at least 100 amino acid residues in length and lacks a B12D5 binding epitope located within the central basic region of the GP64 protein. The genome of the recombinant baculovirus comprises a transgene encoding a fusion protein that comprises a signal peptide, the heterologous antigen, and the C-terminal region of the baculovirus envelope GP64 protein, in which the antigen is located between the signal peptide and the C-terminal region of the GP64 protein. The recombinant baculovirus is for use in eliciting an antigen-specific immunogenic response in a subject in need thereof.本發明提供一種於其外膜上展現一融合蛋白之基因重組桿狀病毒。該融合蛋白包含一異種抗原以及桿狀病毒外膜套GP64蛋白之C端區域，其具有至少100個胺基酸殘基之長度，且缺乏位於GP64蛋白中央鹼性區域內之B12D5結合決定位。該基因重組桿狀病毒之基因組包含一編碼一融合蛋白之轉基因，該融合蛋白包含一訊號胜肽、該異種抗原，及該桿狀病毒外膜套GP64蛋白之C端區域，其中該抗原係位於該訊號胜肽與該GP64蛋白C端區域之間。基因重組桿狀病毒係供用於在需要治療之個體體內誘發一抗原特異性免疫原性反應。
