Chang, Tsewen,Chen, Jiun-bo,Wu, Pheidias,Hung, Alfur F.
申请号：
AU2014203008
公开号：
AU2014203008B2
申请日:
2014.06.03
申请国别(地区):
AU
年份:
2016
代理人:
摘要：
#$%^&*AU2014203008B220160331.pdf#####ANTI-CEmX ANTIBODIES CAPABLE OF BINDING TO HUMAN mIgE ON B LYMPHOCYTES Abstract The invention pertains to the generation and utility of antibodies that can bind effectively to CEmX domain on membrane-bound IgE (mlgE) expressed on the surface of human B lymphocytes. The CEmX domain of 52 amino acid residues, located between the CH4 domain and the C-terminal membrane-anchor peptide on human membrane-bound epsilon chain, had been suggested as an antigenic site for immunological targeting of B cells expressing mlgE. Previous reported monoclonal antibodies, including a20, which bind to RADWPGPP peptide at the C-terminal of CEmX, have now been found to bind poorly to mlgE on human B cells. We have discovered that only monoclonal antibodies specific for certain segments, such as GLAGGSAQSQRAPDRVL and HSGQQQGLPRAAGGSVPHPR, of CEmX can bind effectively to mlgE on human B cells and hence have the utility for targeting those B cells for the treatment of diseases mediated by IgE.
