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PRIMED CARDIAC PROGENITORS AND METHODS FOR MAKING AND USING SAME
专利权人:
WISCONSIN ALUMNI RESEARCH FOUNDATION
发明人:
BIERMANN, MITCH JAMES,KAMP, TIMOTHY JOSEPH
申请号:
CA3036233
公开号:
CA3036233A1
申请日:
2017.09.29
申请国别(地区):
CA
年份:
2018
代理人:
摘要:
An improvement to the GiWi protocol for differentiating human pluripotent cells to developmentally mature cardiomyocytes includes a step of activating innate immunity in mesoderm stage cells in the in vitro differentiation culture. When the mesoderm cells, which are precursors to cardiac progenitor cells, are primed by exposure to an activator of innate immunity, a population of cardiomyocytes is generated that is more developmentally mature than is generated in the GiWi protocol without the primed step. Also provided herein are in vitro ventricular conductive microtissues and isolated, in vitro populations of ventricular conduction system-like cells and methods for making the same.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/
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