The present invention relates to the field of biological sample testing technology, and in particular, to a biological sample reaction vessel. A reagent storage portion and a push rod movable relative to the reagent storage portion are packaged in the reaction vessel; the reagent storage portion comprises at least one reagent containing cavity, and the reagent containing cavity is sealed by a sealing element; and the push rod is connected to the sealing element, and the push rod is used for cooperation with an external device to separate the sealing element from the reagent storage portion. In this application, the reagent storage portion and the push rod are both packaged in the biological sample reaction vessel, and in reaction, the biological sample reaction vessel only needs to cooperate with a test cassette. With one operation, that is, inserting the biological sample reaction vessel into the external device, the reagent in the reagent storage portion can be released rapidly.