The present invention provides a protein-adjuvant vaccine with heat resistance, which is designed from wild-type chicken interleikin-1β to construct a new chicken interleikin-1β, named CP- interleikin-1β. The CP- interleikin-1β having improved heat resistance keeps the original biological activity, and which helps to develop avian interleikin-1β vaccine adjuvant with high efficiency and uses in medical application. The invention also provides a method of producing such protein-adjuvant vaccine.本發明提供一種具耐熱性的蛋白質疫苗佐劑,其是將野生型雞的介白素-1β胺基酸序列進行設計改良,而建構出一個新型雞的介白素-1β,將其命名為環形重組介白素-1β蛋白質。該重組介白素-1β蛋白質對熱的耐受性明顯提升,同時也兼具原有蛋白質的生物活性,其有助於開發高效能的禽類介白素-1β疫苗佐劑並應用於醫療等用途。本發明亦提供一種製備該耐熱性的蛋白質疫苗佐劑之方法。
