James Giulianotti,Dorothea Reilly,Kieran Aurori,Laura C. Simmons
申请号：
US14934094
公开号：
US10066002B2
申请日:
2015.11.05
申请国别(地区):
US
年份:
2018
代理人:
摘要：
Provided herein are methods of producing a recombinant polypeptide containing two chains, such as an immune mobilizing monoclonal T-cell receptor against cancer (ImmTAC) protein including an alpha chain and a beta chain. In particular, methods are provided for producing heterologous secretory proteins in bacteria through utilization of optimized expression vectors and culture processes.
