A chest diagnostic support information generation system includes: a radiography section which radiographs a chest portion of a subject an image analysis section which generates a diagnostic support information based on an image data generated by the radiography section and a display which displays the diagnostic support information, wherein the radiography section obtains a plurality of frame images which show a motion state of the chest of the subject, and the image analysis section includes: a breathing information generation section which generates the diagnostic support information relating to breathing of the subject based on a difference value of the pixel or the block corresponded with each other between image frames temporally adjacent among the plurality of frame images and a blood flow information generation section which generates the diagnostic support information relating to blood flow of the subject based on a generated output signal wave form.