BURGESS, COLLEEN M.,伯吉斯 柯琳 M,伯吉斯 柯琳 M,CHAPPEL, SCOTT,查佩尔 斯考特,查佩爾 斯考特,TZIANABOS, ARTHUR O.,载纳伯斯 亚瑟 O,載納伯斯 亞瑟 O
申请号：
TW104141020
公开号：
TW201629084A
申请日:
2015.12.08
申请国别(地区):
TW
年份:
2016
代理人:
摘要：
Preparations and methods of producing a concentrated mitochondrial preparation using inverted phase centrifugation are disclosed. The methods include centrifuging a closed-bottom tube containing a heavy phase including a mitochondrial suspension in the portion of the tube proximal to the axis of rotation and a light phase in the portion of the tube distal to the axis of rotation. The centrifugation causes the suspension to form a concentrated mitochondrial layer at the interface between the heavy layer and the light layer, and the fluid at this interface is collected to produce the concentrated mitochondrial preparation. The concentrated mitochondrial preparation can be used for various applications, including in vitro fertilization applications.本發明揭示使用倒置相離心生產濃縮線粒體製劑的製劑和方法。所述方法包括:離心底部閉合的管,所述管含有位於所述管的旋轉軸的近端部分的包含線粒體懸浮液的重相以及位於所述管的旋轉軸的遠端部分的輕相。離心使得所述懸浮液在重層與輕層之間的介面處形成濃縮線粒體層,收集此介面處的流體以生產所述濃縮線粒體製劑。所述濃縮線粒體製劑可用於各種應用,包括體外受精應用。110‧‧‧管120‧‧‧水層130‧‧‧有機層140‧‧‧密封180‧‧‧緩衝材料210‧‧‧管
