An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. Also provided is recombinant PCV2 ORF2 protein, and immunogenic compositions comprising PCV2 ORF2 protein. Moreover, multivalent combination vaccines are provided which include an immunological agent effective for reducing the incidence of or lessening the severity of PCV2 infection, preferably PCV2 ORF2 protein, or an immunogenic composition comprising PCV2 ORF2 protein, and at least one immunogenic active component of another disease-causing organism in swine,本發明係提供一種回收由得自第二型豬環狀病毒之開放編閱架構2表現之蛋白質的新穎方法。亦提供重組的PCV2 ORF2蛋白質和包括PCV2 ORF2蛋白質的免疫組合物。此外,亦提供多價混合疫苗,其包括可有效降低PCV2感染之發生率或減少其嚴重性的免疫學製劑,較佳的是PCV2 ORF2蛋白質,或包括PCV2 ORF2蛋白質之免疫組合物,以及至少一種引起其他豬病之生物的免疫活性組份。
