PROBLEM TO BE SOLVED: To provide a plant cell differentiation promoter promoting differentiation from a callus to a normal adventitious embryo, or promoting differentiation of an adventitious root or adventitious bud from a plant cutting, and as a result, enabling to stably obtain a regenerated plant.SOLUTION: There is provided a plant differentiation promoter comprising as an active ingredient a specific ketol fatty acid or a derivative thereof. The promotor comprises a 4-24C ketol fatty acid whose carbon atom composing a carbonyl group and carbon atom bonded with a hydroxy group are positioned at α position.COPYRIGHT: (C)2013,JPO&INPIT【課題】本発明は、カルスからの正常な不定胚への分化を促進し、又は植物切片からの不定根又は不定芽の分化を促進し、結果として安定して再生植物体を得ることを可能にする植物細胞分化促進剤を提供することを課題とする。【解決手段】本発明の特定のケトール脂肪酸、又はその誘導体を有効成分として含有させることによる。【選択図】図2