A prosthetic heart valve using a pericardium is disclosed where: three hetero-biological tissue slices, which are extracted in a quadrangular shape from a bovine pericardium or a porcine pericardium, are prepared； each hetero-biological tissue slice is folded so as to form an inner tissue slice inside and an outer tissue slice outside, the outer tissue slice being longer than the inner tissue slice； each of the inner tissue slice and outer tissue slice is coupled by sewing in a semicircular shape with a first coupling thread such that each inner tissue slice forms a pulmonic valve slice inside the first coupling thread and a fixing slice outside the first coupling thread such that each hetero-biological tissue slice is formed in a cylindrical shape by allowing each slice to be adjacent to each other. The disclosed prosthetic heart valve has increased durability and prevents reverse blood flow.
